Somatic embryogenesis and plant regeneration inMedicago suffruticosa |
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Authors: | Xiu-Oing Li Yves Demarly |
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Affiliation: | (1) Laboratoire d'Amélioration des Plantes, Université de Paris-Sud-XI, 91405 Orsay cedex, France;(2) Present address: Department of Biology, McGill University, 1205 Dr. Penfield Ave., H3A 1B1 Montreal, PQ, Canada |
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Abstract: | A successful procedure has been designed for the regeneration of plantlets from leaf sections of the self-pollinating species,Medicago suffruticosa. Callus growth was promoted by a 4-week culture period on liquid Kao's medium containing 4.9 M benzyladenine and 4.5 M 2,4-dichlorophenoxyacetic acid (2,4-d), followed by a 4-day treatment in which the benzyladenine was elevated to 44.4 M. Shoots/plantlets were observed after 3–4 weeks culture on growth regulator-free agar-solidified medium. Under these conditions, the regeneration frequency from callus was 18% and a histological study showed that this regeneration was through somatic embryogenesis. The growth regulator treatment, with a relatively high concentration of growth regulators (44.4 M benzyladenine) for a short time period (4 days), is important for inhibiting polyphenol compounds and for stimulating callus growth and plant regeneration.Abbreviations 2,4-d 2,4-dichiorophenoxyacetic acid - BA benzyladenine - NAA -napthaleneacetic acid |
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Keywords: | growth regulator treatment self pollinating alfalfa |
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