Ceruloplasmin stimulates NADH oxidation of pig liver plasma membrane. |
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| Authors: | F J Alcaín J M Villalba H L?w F L Crane P Navas |
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| Affiliation: | Departamento de Biología Celular, Universidad de Córdoba, Spain. |
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| Abstract: | NADH oxidation by pig liver plasma membranes is stimulated by ceruloplasmin (CUP) reaching a maximal value at 50 U/ml of CUP. NADH oxidation activated by CUP is proportional to the amount of protein. Concanavalin A (Con A) which recognizes the glucidic residues of the CUP required for binding to the receptor inhibits the NADH oxidation in a dose-responsive manner. Both adriamycin and bathophenantroline disulfonate (BPS), previously reported as transplasma membrane electron transport inhibitors, also inhibit the CUP-stimulated NADH oxidation of pig liver plasma membranes. Our results show a clear interaction between CUP and the NADH oxidase of plasma membrane, which supports an oxidative role for CUP in its growth effect. |
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