| 细胞电穿孔导入DNA的动态特征 |
| |
| 引用本文： | 汪和睦, 李金慧,.细胞电穿孔导入DNA的动态特征[J].微生物学通报,1994,21(2):67-71. |
| |
| 作者姓名： | 汪和睦 李金慧 |
| |
| 作者单位： | 南开大学物理系; |
| |
| 摘 要： | 本文以Ｅ．ＣｏｌｉＨＢ１０１为模型细胞，研究了不同幅度和时间常数的ＲＣ放电脉冲条件下，触发细胞穿孔，导入长度分别为４２２和１６８０ｎｍ的超螺旋长丝形质粒ｐＵＣ－１８和ｐＣＰ１０，及精胺缩合后直径为８８ｎｍ的复曲面体的质粒ｐＣＰ１０，测定了相应的细胞转化率。实验结果显示了细胞电穿孔导入ＤＮＡ的动态特征。
|
| 关 键 词： | 细胞电穿孔 E．Coli 质粒DNA转化 DNA缩合 |
THE DYNAMIC CHARACTER OF INTRODUCTION OF DNA INTO CELLS BY ELECTROPORATION |
| |
| Wang Hemu, Li Jinhui.THE DYNAMIC CHARACTER OF INTRODUCTION OF DNA INTO CELLS BY ELECTROPORATION[J].Microbiology,1994,21(2):67-71. |
| |
| Authors: | Wang Hemu Li Jinhui |
| |
| Abstract: | In this study E. coli HB101 is used as the cell model. It has been investigated to causecell electroporation with RC electric pulses of different strength and different width andintroduce various kinds of plasmids into cells. These plasmids contain the long thready closedcircular plasmids of pUC-18, pCP10,which are 422nm and 1680nm long respectively, and theplasmid of pCP10 condensed with spermine, which is a kind of toroidal particle with the diameterof 88nm, Their transformation efficiencies have been mensured respectively. The experimentalresults manifest the dynamic character of introduction of DNA into cells by electroporation. |
| |
| Keywords: | Cell electroporation E coli Plasmid transformation DNA condensation |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
