| 犬细小病毒单克隆抗体的制备及其与免疫血清在血凝抑制中的比较 |
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| 引用本文： | 符兆英, 渠川玫, 田克恭, 朱关福,. 犬细小病毒单克隆抗体的制备及其与免疫血清在血凝抑制中的比较[J]. 微生物学通报, 1994, 21(2): 88-92 |
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| 作者姓名： | 符兆英 渠川玫 田克恭 朱关福 |
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| 作者单位： | 北京军事医学科学院实验动物中心; 北京军事医学科学院微生物研究所; |
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| 摘 要： | 将用犬细小病毒免疫的ＢＡＬＢ／ｃ小鼠的脾细胞与Ｓｐ２／０骨髓瘤细胞在聚乙二醇作用下融合，经筛选、克隆，得到６株稳定分泌犬细小病毒单克隆抗体的杂交瘤。用杂交瘤腹水作血凝抑制试验，检测分别含有犬细小病毒、猫泛白细胞减少症病毒和水貂肠炎病毒的标本，及ＣＰＶ感染犬粪便标本，并与免疫血清作对比。结果表明，用单克隆抗体作血凝抑制试验，比用免疫血清作具有特异性高、操作简便省时等优点，而二者敏感性一致。
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| 关 键 词： | 犬细小病毒 单克隆抗体 血凝抑制试验 |
PREPARATION OF MONOCLONAL ANTIBODIES (McAbs)AGAINST CANINE PARVOVIRUS AND COMPARISION BETWEEN MCABS AND SERA IN HEMAGGLUTINATION INHIBITION |
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| Fu Zhaoying,Qu Chuanmei,Tian Kegong. PREPARATION OF MONOCLONAL ANTIBODIES (McAbs)AGAINST CANINE PARVOVIRUS AND COMPARISION BETWEEN MCABS AND SERA IN HEMAGGLUTINATION INHIBITION[J]. Microbiology China, 1994, 21(2): 88-92 |
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| Authors: | Fu Zhaoying Qu Chuanmei Tian Kegong |
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| Abstract: | Spleen cells from a BALB/c mouse immunized with canine parvovirus (CPV) werefused with Sp2/0 myeloma cells by polyethylene glycol, After screening and cloning, 6 hybridcell lines steadily secreting monoclonal antibodies (McAb) were obtained. Hybridoma asciteswere used in hemagglutination inhibition (HI) test to detect samples known containing CPV,feline panleukopenie virus (FPLV),and mink enteritis virus (MEV) respectively, and fecalsamples from CPV infected dogs, compared with anti-CPV sera. The results suggested that HItest with CPV-McAbs was more specific and time-saving than that with anti-CPV sera, yet bothwere equally sensitive. |
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| Keywords: | Canine parvovirus Monoclonal antibody Hemagglutination inhibition test |
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