| 血沉标本中人巨细胞病毒DNA的检出 |
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| 引用本文： | 孟潞英, 张文炳, 彭华国, 董自正, 石成华,.血沉标本中人巨细胞病毒DNA的检出[J].微生物学通报,1994,21(1):26-30. |
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| 作者姓名： | 孟潞英 张文炳 彭华国 董自正 石成华 |
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| 作者单位： | 第一军医大学微生物学教研室; 军事医学科学院生物工程研究所; |
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| 摘 要： | 用聚合酶链反应（ＰＣＲ）和地高辛标记探针（ｄｉｇ－ｐｒｏｂｅ）检测临床血沉标本中人巨细胞病毒（ＨＣＭＶ）ＤＮＡ。结果表明，人群血标本中ＨＣＭＶ－ＤＮＡ携带率较高；ＰＣＲ技术较ｄｉｇ－ｐｒｏｂｅ更敏感、快速、简便，二者检出ＨＣＭＶ－ＤＮＡ阳性率分别为８３．３％和６０．３％；ＨＣＭＶ－ＤＮＡ检出率、ＨＣＭＶ－ＩｇＭ检出率与血沉值高低之间无相关性。
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| 关 键 词： | 人巨细胞病毒（HCMV） 聚合酶链反应（PCR） 血沉标本 |
DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN CLINICAL BLOOD SPECIMENS OF DETERMINING ERYTHROCYTE SEDIENTATION RATE |
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| Meng Luying,Zhang Wenbing,Peng Huaguo.DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN CLINICAL BLOOD SPECIMENS OF DETERMINING ERYTHROCYTE SEDIENTATION RATE[J].Microbiology,1994,21(1):26-30. |
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| Authors: | Meng Luying Zhang Wenbing Peng Huaguo |
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| Abstract: | Human cytomegalovirus(HCMV)DNA in 78 clinical blood specimens of determining erythrocyte sedimentation rate(ESR)wasdetected by polymerase chain reaction(PCR)and digoxigenin labelled probe(dig-probe).Results showed that PCR was moresensitive,simple and high-speed than dig-probe,The positive percentages of HCMV DNAdetermined by PCR and dig-probe were 83.3%and 60.3%respectively, In addition,there wasnot any relation between HCMV DNA or Igm positive rate and ESR. |
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| Keywords: | Human Cytomegalovirus(HCMV) Polymerase Chain Reaction(PCR) BloodSpecimens |
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