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A dual-purpose synthetic colloidal platform for protease mapping: substrate profiling for Dengue and West Nile virus proteases |
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| Authors: | Marcon Lionel Kozak Darby Battersby Bronwyn J Chappell Keith J Fairlie David P Young Paul Trau Matt |
| Institution: | a Nanotechnology and Biomaterials Centre, Level 5 East, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Saint Lucia, QLD 4072, Australia b School of Molecular and Microbial Sciences, University of Queensland, Saint Lucia, QLD 4072, Australia c Institute for Molecular Bioscience, University of Queensland, Saint Lucia, QLD 4072, Australia |
| Abstract: | In a proof of concept study, we created a small focused fluorescent hexapeptide library onto 14 multiplexed barcoded sets of silica particles to probe the substrate recognition specificity of West Nile and Dengue virus proteases. A flow cytometric analysis demonstrated that the optical signature of each bead population remained distinguishable throughout the solid-phase peptide synthesis and proteolytic assay. As expected, both proteases displayed a narrow specificity for lysine and arginine residues in the P1 and P2 substrate positions. This open-ended platform enables the fast and simultaneous identification of peptide substrates and is applicable to other proteases. |
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