Siderophore production by actinomycetes isolates from two soil sites in Western Australia |
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Authors: | Joanna Lee Armin Postmaster Hooi Peng Soon David Keast Kerry C Carson |
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Institution: | (1) Discipline of Microbiology and Infectious Diseases, PathWest Laboratory Medicine WA, QEII Medical Center, Hospital Ave, Nedlands, WA, Australia;(2) Actinogen Ltd., Level 2, 231 Adelaide Tce, Perth, WA, Australia;(3) Discipline of Microbiology and Immunology, School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, 35 Stirling Hwy, Crawley, WA, Australia; |
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Abstract: | The actinomycetes are metabolically flexible soil micro-organisms capable of producing a range of compounds of interest, including
siderophores. Siderophore production by actinomycetes sampled from two distinct and separate geographical sites in Western
Australia were investigated and found to be generally similar in the total percentage of siderophore producers found. The
only notable difference was the proportion of isolates producing catechol siderophores with only 3% found in site 1 (from
the north-west of Western Australia and reportedly containing 40% magnetite) and 17% in site 2 (a commercial stone fruit orchard
in the hills east of Perth with a soil base ranging from sandy loam to laterite). Further detailed characterization of isolates
of interest identified a Streptomyces that produced extracellularly excreted enterobactin, the characteristic Enterobacteriaceae siderophore, and also revealed
some of the conditions required for enterobactin production. Carriage of the entF gene, which codes for the synthetase responsible for the final assembly of the tri-cyclic structure of enterobactin, was
confirmed by PCR in this isolate. Another separate Streptomyces produced a compound that matched the UV/VIS spectra of heterobactin, a siderophore previously only described in Rhodococcus and Nocardia. |
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