The use of ferromagnetic Dacron as solid-phase in chemiluminescent assays |
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| Authors: | S.M.B. Pinheiro L.B. Carvalho Jr. M.E.C. Chaves |
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| Affiliation: | (1) Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami-LIKA, Universidade Federal de Pernambuco, Cidade Universitária, CEP, 50670-910 Recife, PE, Brasil |
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| Abstract: | Ferromagnetic Dacron hydrazide particles (63–74  m) were activated either by converting hydrazide groups to azide or binding glutaraldehyde to the hydrazide groups. Human serum albumin (HSA) was immobilised to the activated matrices and antibodies against HSA were detected by using the chemiluminescent assay. The use of these particles (either Dacron-azide or Dacron-glutaraldehyde) gave similar results compared with the paramagnetic particles commercially available from Ciba Corning. |
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| Keywords: | chemiluminescence Dacron ferromagnetic Dacron human serum albumin magnetic particles |
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