硫磺菌原变种液体培养代谢物生物活性分析

硫磺菌原变种Laetiporus sulphureusvar.sulphureus在液体培养条件下对果蝇具有致死效应,研究发现在液体培养过程中分泌到细胞外的代谢产物是致死效应的主要原因,并且上清液对果蝇的生物活性受pH值的影响。离子交换树脂柱和高效液相色谱分离分析表明草酸存在于硫磺菌原变种培养液的上清液中并且是果蝇致死效应和培养体系pH下降的一个重要因素。硫磺菌原变种在气升式反应器ALR/ff培养体系中草酸的浓度、菌丝体量和pH值呈简单相关。进一步分析发现还有另外一种结构未知、在碱性条件下呈紫红色的色素也具有致死效应。

Bioactivity of Laetiporus sulphureus var. Sulphureus metabolites in liquid culture

The liquid culture of Laetiporus sulphureus var. sulphureus was lethal against fruit fly. It was found that extracellular metabolites were primary causation of the lethal effect against fruit fly, which was influenced by pH value. Isolation and analysis with ion-exchange resin column chromatography and HPLC demonstrated that oxalic acid was present in supernatant of Laetiporus sulphureus var. sulphureus, and it was one of the contributing factors to lethal effect against fruit fly and decrease of pH value of culture system. When cultured in airlift reactor, concentration of oxalic acid, quantity of mycelia and pH value was correlated with each other. Further analysis on elution revealed that a kind of oligidic pigment of amaranth in alkaline condition were also lethal against fruit fly.