Whole-mount in situ hybridization of small invertebrate embryos using laboratory mini-columns |
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Authors: | Irvine Steven Q |
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Institution: | Biological Sciences Center, University of Rhode Island, Kingston, RI 02881, USA. steven.irvine@uri.edu |
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Abstract: | To facilitate the handling of small invertebrate embryos during whole-mount in situ hybridization, a method of solution exchange using laboratory mini-columns was developed. This protocol speeds time-consuming aspiration of buffers, and avoids accidental loss of the embryos, by gently pushing solutions through the column using air pressure from a syringe after each incubation. The next buffer is then added using a pipettor. Embryos are retained on a filter within the column. As many columns as desired may be processed in parallel for different probes or stages. |
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