Neuroprotective activity and cytotoxic potential of two Parmeliaceae lichens: Identification of active compounds |
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| Affiliation: | 1. Department of Pharmacology, Faculty of Pharmacy, University Complutense of Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain;2. Department of Plant Biology II, Faculty of Pharmacy, University Complutense of Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain;1. SONAS, EA921, Universtiy of Angers, SFR QUASAV, Faculty of Health Sciences, Department of Pharmacy, 16 Bd Daviers, 49045, Angers, France;2. Université Rennes, CNRS, ISCR – UMR 6226, F-35000 Rennes, France;3. BioCIS, Université Paris-Sud, CNRS, Université Paris-Saclay, 5 Rue J.-B. Clément, 92290 Châtenay-Malabry, France;4. Université d''Angers, MITOVASC Institute, CarMe team, INSERM U1083, CNRS UMR6015, CARFI facility, 3 rue Roger Amsler, 49100 Angers, France;5. Université de Caen Normandie, Centre d''Etudes et de Recherche sur le Médicament de Normandie (CERMN), 14000 Caen, France;6. University of Vienna, Department of Analytical Chemistry, Währinger Straße 38, 1090 Vienna, Austria;7. University of Vienna, Department of Pharmaceutical Chemistry, Althanstraße 14, 1090 Vienna, Austria;8. University of Vienna, Department of Pharmacognosy, Althanstraße 14, 1090 Vienna, Austria;9. University of Innsbruck, Institute of Pharmacy/Pharmacognosy, Center for Chemistry and Biomedicine, Innrain 80 - 82/IV, 6020 Innsbruck, Austria;1. Chemical Biology, CSIR- Indian Institute of Chemical Technology, Tarnaka, Uppal Road, Hyderabad 500007, India;2. PNSCM-UMRCNRS 6226, ISCR, Faculté des Sciences Pharmaceutiques et Biologiques, Université Européenne de Bretagne, Université de Rennes 1, 2 Av. du Pr Léon Bernard, 35043 Rennes Cedex, France |
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| Abstract: | BackgroundLichens are symbiotic organisms capable of producing unique secondary metabolites, whose pharmacological activities are attracting much interest.PurposeThe present study aimed to investigate the in vitro neuroprotective effects and anticancer potential of methanol extracts of two Parmeliaceae lichens: Cetraria islandica and Vulpicida canadensis. The chemical composition of the two lichens was also determined.MethodsNeuroprotective activity was studied with respect to the antioxidant properties of the extracts; radical scavenging tests (ORAC and DPPH assays) were performed and oxidative stress markers (intracellular ROS production, caspase-3 activity, MDA and glutathione levels) were assessed in a hydrogen peroxide-induced oxidative stress model in astrocytes. Cytotoxic activity was tested against human HepG2 (hepatocellular carcinoma) and MCF-7 (breast adenocarcinoma) cell lines.ResultsCell viability studies identified a single concentration for each extract that was subsequently used to measure oxidative stress markers. Lichen extracts were able to reverse the oxidative damage caused by hydrogen peroxide, thus promoting astrocyte survival. Both lichen extracts also had anticancer activity in the cell lines, with IC50 values of 19.51–181.05 µg/ml. The extracts had a high total phenolic content, and the main constituents identified by HPLC were fumarprotocetraric acid in Cetraria islandica, and usnic, pinastric and vulpinic acids in Vulpicida canadensis. The biological activities of the lichen extracts can be attributed to these secondary metabolites.ConclusionThe lichen species studied are promising sources of natural compounds with neuroprotective activity and cytotoxic potential, and warrant further research. |
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