Oleacein enhances anti-inflammatory activity of human macrophages by increasing CD163 receptor expression |
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| Institution: | 1. Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland;2. Department of Pharmacogenomics, Division of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland;1. Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan;2. Department of Molecular and Cellular Oncology, University of Texas, MD Anderson Cancer Center, TX 77030, USA;3. Institute of Nutrition, China Medical University, Taichung 40402, Taiwan;4. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan;5. Institute of Medicine, Chun Shan Medical University, Taichung 40201, Taiwan;1. Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain;2. Department of Pharmacology and IDM, University of Valencia, 46100 Burjasot, Valencia, Spain;3. Department of Organic Chemistry, Faculty of Chemistry, University of Seville, 41012 Seville, Spain |
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| Abstract: | BackgroundOleacein (dialdehydic form of decarboxymethyl elenolic acid linked to hydroxytyrosol; 3,4-DHPEA-EDA) have been proven to possess antioxidant and anti-inflammatory activity.PurposeIn this study, we examined whether oleacein could increase CD163 and IL-10 receptor expression as well as HO-1 intracellular secretion in human macrophages.MethodsEffect of oleacein (10 and 20 μmol/l) or oleacein together with complexes of haemoglobin (Hb) and haptoglobin 1-1 (Hp11) or haptoglobin 2-2 (Hp22) on expression of IL-10 and CD163 receptor was determined by Flow Cytometry. Expression of CD163mRNA was measured by real-time quantitative RT-PCR. Heme oxygenase 1 (HO-1) intracellular secretion in macrophages was investigated by enzyme-linked immunosorbent assay (ELISA).ResultsOleacein (OC) together with complexes HbHp11 or HbHp22 stimulated the expression of CD163 (30-100-fold), IL-10 (170-300-fold) and HO-1 secretion (60-130-fold) after 5 days of coincubation. The 2-fold (24 h), 4-fold (48 h) increase of CD163 mRNA level and its final (72 h) decrease was also observed.ConclusionOur results suggested that oleacein enhances anti-inflammatory activity of complexes haemoglobin with haptoglobin 1-1 and 2-2 and could play a potential role in the prevention of inflammatory disease related to atherosclerosis. |
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