A comparative analysis on the binding characteristics of various mammalian
albumins towards a multitherapeutic agent,pinostrobin |
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Authors: | Shevin R FEROZ Rumana A SUMI Sri NA MALEK Saad TAYYAB |
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Institution: | Biomolecular Research Group, Biochemistry Programme, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia |
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Abstract: | The interaction of pinostrobin (PS), a multitherapeutic agent with serum albumins of
various mammalian species namely, goat, bovine, human, porcine, rabbit, sheep and dog was
investigated using fluorescence quench titration and competitive drug displacement
experiments. Analysis of the intrinsic fluorescence quenching data revealed values of the
association constant, Ka in the range of 1.49 – 6.12 ×
104 M−1, with 1:1 binding stoichiometry. Based on the PS–albumin
binding characteristics, these albumins were grouped into two classes. Ligand displacement
studies using warfarin as the site I marker ligand correlated well with the binding data.
Albumins from goat and bovine were found to be closely similar to human albumin on the
basis of PS binding characteristics. |
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Keywords: | animal model drug– protein interaction fluorescence spectroscopy mammalian albumin pinostrobin |
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