Effect of hypochlorous acid solution on the eradication and prevention of
Pseudomonas aeruginosa infection,serum biochemical variables,and
cecum microbiota in rats |
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| Authors: | Kazuo GOTO Eri KUWAYAMA Ryoko NOZU Masami UENO Nobuhito HAYASHIMOTO |
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| Affiliation: | 1)Department of Clinical Laboratory Medicine, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan;2)Central Institute for Experimental Animals, 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan |
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| Abstract: | In this study, hypochlorous acid solution, a weak acid, provided as drinking water torats, was evaluated for its ability to eradicate and prevent Pseudomonasaeruginosa infection, while monitoring its simultaneous effect on serumbiochemical variables and microbiota in the rat cecum. The results suggest that thesolution could not eliminate the bacteria in the experimentally infected rats; however,the administration of a 10-parts-per-million (ppm) hypochlorous acid solution as drinkingwater was effective in inhibiting horizontal spread of P. aeruginosainfection among cage mates. Additionally, exposure to hypochlorous solution did not haveany effect on serum biochemical variables of the rat including levels of totalcholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkalinephosphatase (ALP), albumin, total bilirubin, lipase, amylase, urea nitrogen, totalprotein, calcium (Ca), phosphorus (P), sodium (Na), chlorine (Cl), except for potassium(K) levels. The most frequently isolated bacteria in the rat cecum included speciesbelonging to Bacteroidales, Lactobacillus,Clostridiales, Erysipelotrichaceae,Akkermansia, Coriobacteriales, andFirmicutes. The ratio of the terminal restriction fragment lengthpolymorphism (T-RFLP) peaks did not differ across rats administered with 5 and 10 ppm weakacid solution as compared to the control group for any of the bacteria, except forErysipelotrichaceae and Firmicutes, where the ratio ofT-RFLP peaks was higher in the 5 ppm group for Erysipelotrichaceae and inthe 10 ppm group for Firmicutes than that in the control group(P<0.01). The results suggest that the weak acid hypochloroussolution could not eradicate P. aeruginosa completely from rats. Thesolution was effective in preventing infection without affecting serum biochemicalvariables; however, some of bacterial microbiota may have changed due to administration ofthe solution. |
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| Keywords: | microbiota Pseudomonas aeruginosa rat T-RFLP weak acid hypochlorous solution |
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