Alterations in brush border membrane enzymes,carbohydrate metabolism and oxidative damage to rat intestine by potassium bromate |
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Authors: | Mir Kaisar Ahmad Aijaz Ahmed Khan Riaz Mahmood |
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Institution: | 1. Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India;2. Department of Anatomy, Faculty of Medicine, J. N. Medical College, Aligarh Muslim University, Aligarh 202002, U.P., India |
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Abstract: | The acute toxicity of potassium bromate (KBrO3) on rat small intestine was studied in this work. Animals were given a single oral dose of KBrO3 (100 mg/kg body weight) and sacrificed 12, 24, 48, 96 and 168 h after the treatment; control animals were not given KBrO3. The administration of KBrO3 resulted in a reversible decline in the specific activities of several BBM enzymes. Lipid peroxidation, protein oxidation and hydrogen peroxide levels increased while total sulfhydryl groups and reduced glutathione decreased in KBrO3-treated rats indicating induction of oxidative stress in the intestinal mucosa. The activities of anti-oxidant and carbohydrate metabolic enzymes were also altered upon KBrO3 treatment. The maximum changes in all the parameters were 48 h after administration of KBrO3 after which recovery took place, in many cases almost to control values after 168 h. Histopathological studies supported the biochemical findings showing extensive damage to the intestine at 48 h and recovery at 168 h. These results show that a single oral dose of KBrO3 causes reversible oxidative damage to the intestine. |
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Keywords: | Anti-oxidant Brush border membrane Carbohydrate metabolism Intestine Potassium bromate |
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