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A simple,cost-effective and flexible method for processing of snap-frozen tissue to prepare large amounts of intact RNA using laser microdissection |
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| Authors: | Phulwinder K Grover Adrian G Cummins Timothy J Price Ian C Roberts-Thomson Jennifer E Hardingham |
| Institution: | 1. Departments of Surgery, Basil Hetzel Institute for Translational Health Research, The Queen Elizabeth Hospital and the Discipline of Medicine, University of Adelaide, 28 Woodville Road, Woodville South, South Australia 5011, Australia;2. Department of Gastroenterology and Hepatology, The Queen Elizabeth Hospital and the Discipline of Medicine, University of Adelaide, 28 Woodville Road, Woodville South, South Australia 5011, Australia;3. Department of Haematology–Oncology, The Queen Elizabeth Hospital and the Discipline of Medicine, University of Adelaide, 28 Woodville Road, Woodville South, South Australia 5011, Australia |
| Abstract: | Understanding the molecular basis of disease requires gene expression profiling of normal and pathological tissue. Although the advent of laser microdissection (LMD) has greatly facilitated the procurement of specific cell populations, often only small amounts of low quality RNA is recovered. This precludes the use of global approaches of gene expression profiling which require sizable amounts of high quality RNA. Here we report a method for processing of snap-frozen tissue to prepare large amounts of intact RNA using LMD. |
| Keywords: | Laser microdissection RNA integrity High quality RNA Molecular profiling Cells of interest Tissue heterogeneity |
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