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| 右旋磷霉素生物转化菌株的筛选和鉴定 | |
| 引用本文： | 胡芳欣,胡江春,徐慧,潘华奇,徐杨. 右旋磷霉素生物转化菌株的筛选和鉴定[J]. 生物学杂志, 2010, 27(2): 21-24. DOI: 10.3969/j.issn.1008-9632.2010.02.021 |
| 作者姓名： | 胡芳欣 胡江春 徐慧 潘华奇 徐杨 |
| 作者单位： | 1. 沈阳药科大学制药工程学院,沈阳,110016 2. 沈阳药科大学制药工程学院,沈阳,110016;中国科学院沈阳应用生态研究所,沈阳,110016 3. 中国科学院沈阳应用生态研究所,沈阳,110016 |
| 基金项目： | 重大新药创制科技专项，辽宁省产学合作技术联盟项目 |
| 摘 要： | 以右旋磷霉素为唯一碳源进行固体初筛和液体复筛,获得生物转化能力较强的32株菌;再以杀卤虫模型筛选具有杀虫活性物质的菌株,得到一株具有较强杀虫活性的真菌FM94,通过对该菌的形态特征观察及ITS序列分析,鉴定为腾仓赤霉（Gibberella fujikuroi）;初步考察其生物转化条件,结果表明在以右旋磷霉素为唯一碳源,初始pH值为3.5,转化培养第4d时转化产物杀卤虫活性最高。 |
| 关 键 词： | 右旋磷霉素 生物转化 筛选 杀虫活性 |
Screening and identifying of strains for dextrofosfomysin biotransformation |
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| HU Fang-xin,HU Jiang-chun,,XU Hui,PAN Hua-qi,XU Yang. Screening and identifying of strains for dextrofosfomysin biotransformation[J]. Journal of Biology, 2010, 27(2): 21-24. DOI: 10.3969/j.issn.1008-9632.2010.02.021 | |
| Authors: | HU Fang-xin HU Jiang-chun XU Hui PAN Hua-qi XU Yang |
| Affiliation: | HU Fang-xin1,HU Jiang-chun1,2,XU Hui2,PAN Hua-qi2,XU Yang2(1.Shenyang Pharmaceutical University,Shenyang 110016,2.Institute of Applied Ecology,Chinese Academy of Science,China) |
| Abstract: | Thirty-two strains were screened with the dextrofosfomysin as the sole carbon source.Insecticidal activity strains were screened by liquid bioassay with brine shrimp.The results showed that a strain named FM94 had high insecticidal activity.Its morphology and internal transcribed spacer (ITS) sequence were studied.The results indicated that the strain FM94 belonged to Gibberella fujikuroi.The optimum transformation condition that produced high insecticidal activity was obtained.Dextrofosfomysin used as the ... |
| Keywords: | dextrofosfomysin biotransformation screening insecticidal activity |
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