Structural analysis of arabinoxylans isolated from native and malted finger millet (Eleusine coracana, ragi) |
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Authors: | Subba Rao M V S S T Muralikrishna G |
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Institution: | Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, Karnataka, India. |
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Abstract: | Structural elucidation of purified arabinoxylans isolated from finger millet and its malt by methylation, GLC-MS, periodate oxidation, Smith degradation, NMR, IR, optical rotation, and oligosaccharide analysis indicated that the backbone was a 1,4-beta-D-xylan, with the majority of the residues substituted at C-3. The major oligosaccharide generated by endo xylanase treatment was homogeneous with a molecular weight of 1865 Da corresponding to 14 pentose residues as determined by MALDI-TOF-MS and gel filtration on Biogel P-2. The structural analysis of this oligosaccharide showed that it contained 8 xylose and 6 arabinose residues, substituted at C-3 (monosubstituted) and at both C-2 and C-3 (disubstituted). |
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Keywords: | Arabinoxylans Finger millet Malt |
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