Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector |
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| Authors: | Elke Kinner,Darko Po&# ta,Sandra Strö er,Horst Schmieger |
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| Affiliation: | Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Str. 1a, D-80638 München, FRG |
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| Abstract: | Abstract The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram-positive bacteria that have been studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus . |
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| Keywords: | Actinophage cos site Cohesive end Transduction |
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