Diet-induced obesity increases NF-κB signaling in reporter mice |
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Authors: | Harald Carlsen Fred Haugen Susanne Zadelaar Robert Kleemann Teake Kooistra Christian A. Drevon Rune Blomhoff |
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Affiliation: | 1Department of Nutrition, The Medical Faculty, Institute of Basic Medical Sciences, University of Oslo, P.O. Box 1046, Blindern 0316 Oslo, Norway ;2Department of Vascular and Metabolic Diseases, TNO-BioSciences, Leiden, The Netherlands |
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Abstract: | The nuclear factor (NF)-κB is a primary regulator of inflammatory responses and may be linked to pathology associated with obesity. We investigated the progression of NF-κB activity during a 12-week feeding period on a high-fat diet (HFD) or a low-fat diet (LFD) using NF-κB luciferase reporter mice. In vivo imaging of luciferase activity showed that NF-κB activity was higher in the HFD mice compared with LFD-fed mice. Thorax region of HFD females displayed fourfold higher activity compared with LFD females, while no such increase was evident in males. In male HFD mice, abdominal NF-κB activity was increased twofold compared with the LFD males, while females had unchanged NF-κB activity in the abdomen by HFD. HFD males, but not females, exhibited evident glucose intolerance during the study. In conclusion, HFD increased NF-κB activity in both female and male mice. However, HFD differentially increased activity in males and females. The moderate increase in abdomen of male mice may be linked to glucose intolerance. |
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Keywords: | High-fat diet Luciferase Inflammation Glucose intolerance Molecular imaging |
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