Neutron diffraction studies of oral stratum corneum model lipid membranes |
| |
Authors: | Natalia Yu Ryabova Stanislav G Sheverev Thomas Hauß |
| |
Institution: | 1. Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, 141980, Moscow Region, Russia 2. Skobeltsyn Institute of Nuclear Physics, Lomonosov Moscow State University, Moscow, Russia 3. Institute Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie, Berlin, Germany
|
| |
Abstract: | In this work we have investigated model lipid mixtures simulating a lipid component of oral stratum corneum (OSC). Neutron diffraction experiments on oriented samples have revealed that SM (bovine brain)/dipalmitoylphosphatidylethanolamine/dipalmitoylphosphatidylcholine (DPPE/DPPC) mixtures at molar ratios of 1/2/1 and 1/1/1 are one-phase membranes. The incorporation of low concentrations of ceramide 6 and cholesterol into SM/DPPC/DPPE bilayers does not result in a phase separation, affecting membrane hydration. The model OSC membrane composed of ceramide 6/cholesterol/fatty acids/cholesterol sulfate/SM (bovine brain)/DPPE/DPPC is characterized by coexistence of several lamellar phases, that behave differently during their hydration in water excess. The phase with lamellar repeat distance of about 45 Å is likely a ceramide-rich phase and shows a restricted swelling in water, while another phase with repeat distance of 50 Å swells very quickly on 15 Å and then disappears. Our results indicate that phospholipid-rich and ceramide-rich domains could possibly coexist in the intercellular space of oral epithelium. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|