Cloning of a gene encoding raw-starch-digesting amylase from a Cytophaga sp. and its expression in Escherichia coli |
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| Authors: | Jeang Chii-Ling Chen Li-Shien Chen Ming-Yu Shiau Rong-Jen |
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| Affiliation: | Department of Food Science, National Chung Hsing University, 250 Kuokang Road, Taichung 402, Taiwan, Republic of China. cljeang@nchu.edu.tw |
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| Abstract: | A raw-starch-digesting amylase (RSDA) gene from a Cytophaga sp. was cloned and sequenced. The predicted protein product contained 519 amino acids and had high amino acid identity to alpha-amylases from three Bacillus species. Only one of the Bacillus alpha-amylases has raw-starch-digesting capability, however. The RSDA, expressed in Escherichia coli, had properties similar to those of the enzyme purified from the Cytophaga sp. |
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