Rapid coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip based mass spectrometry for the identification of proteins in nucleoprotein interactions |
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| Authors: | Bouffartigues Emeline Leh Hervé Anger-Leroy Marielle Rimsky Sylvie Buckle Malcolm |
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| Affiliation: | Enzymologie et cinétique structurale, Laboratoire de Biotechnologies et de Pharmacologie génétique Appliquée, UMR 8113 CNRS, Institut d'Alembert, Ecole Normale Supérieur de Cachan, Cachan, France. |
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| Abstract: | We compared coupling approaches of SPR to LC-MS and ProteinChip-based mass spectrometry (SELDI) as a means of identifying proteins captured on DNA surfaces. The approach we outline has the potential to allow multiple, quantitative analysis of macromolecular interactions followed by rapid mass spectrometry identification of retained material. |
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