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Detection of endo-xylanase activities in electrophoretic gels with congo red staining
Authors:Javier D Breccia  Mario D Baigorf  Guillermo R Castro  Faustino Siñeriz
Institution:(1) Cátedra de Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán - PROIMI, Avda. Belgrano y Caseros, 4000 Tucumán, Argentina
Abstract:Summary A modification of the Congo Red zymographic technique is used to analyse multiple xylanase activities of Bacillus sp. MIR-32 after PAGE and SDS-PAGE. Xylanase activities are associated with at least two major proteins of 18.4 and 19.6 kDa.
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