富油脂微藻育种技术研究进展

微藻因生长迅速、光合作用效率高、分布范围广和油脂积累能力强等优势,已被认为是生产生物柴油的理想原料。诱变育种可改善野生型微藻生长缓慢、油脂含量低和环境适应能力弱等缺陷,提高了以微藻生产生物柴油的可行性。概述了物理、化学和基因工程三类诱变育种方法的研究现状,介绍了低场核磁共振和荧光激活细胞分选两种富油脂微藻筛选技术以及一种测定诱变藻株致死率的方法,讨论了三种诱变方法的应用前景,供相关研究人员参考。

Advances in Fat-Rich Microalgae Breeding Techniques

Microalgae is considered to be the ideal raw material for the production of biodiesel for its rapid growth, high photosynthetic efficiency, wide distribution and strong oil accumulation ability. Mutation breeding can improve the slow growth of wild-type microalgae, the low oil content and the poor ability of environmental adaptability, and improve the feasibility of the production of biodiesel with microalgae. Firstly, the current research status of three kinds of mutagenic breeding methods, such as physical, chemical and genetic engineering, was outlined. At the same time, the screening techniques of low field nuclear magnetic resonance and fluorescence activated cell sorting were introduced. Last but not least the application prospect of three mutagenesis methods were discussed to provide reference for relevant researchers.