Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
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Authors: | Woro Anindito Sri Tunjung Masaki Yokoo Yuko Miyake Eimei Sato |
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Affiliation: | a Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi Aobaku, Sendai 981-8555, Japan b Innovation of New Biomedical Engineering Center, Tohoku University, Sendai 980-8575, Japan |
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Abstract: | We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus-oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA, expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44 antibody decreased the expression of procaspases suggesting that perturbation of HA-CD44 binding leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA-CD44 binding is important on apoptosis inhibitory mechanism in porcine granulosa cells. |
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Keywords: | Hyaluronan CD44 FSH Caspase Apoptosis of granulosa cells |
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