首页 | 本学科首页   官方微博 | 高级检索  
     


Extracellular matrix is required for the survival and differentiation of transplanted hepatic progenitor cells
Authors:Yoshihiko Tsukada  Atsushi Suetsugu  Yosuke Osawa  Hisataka Moriwaki
Affiliation:Department of Gastroenterology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan
Abstract:Engelbreth-Holm-Swarm (EHS) gel has been reported to maintain the mature hepatocyte phenotypes in primary cultured hepatocytes. We investigated the effect of EHS gel on the differentiation of fetal liver cells, which contain stem/progenitor cells. The isolated fetal liver cells cultured on EHS gel formed a spherical shape and increased liver-specific gene expressions compared with cells cultured on collagen. The hepatic progenitor cells that were transplanted subcutaneously to BALB/c nude mice could survive and express hepatocyte marker alpha-fetoprotein when the cells were suspended with EHS gel. These findings demonstrate that EHS gel supports cytodifferentiation from immature progenitor cells to hepatocytes and maintain its differentiated phenotypes in vitro and in vivo.
Keywords:EHS gel, Engelbreth-Holm-Swarm gel   AFP, alpha-fetoprotein   ECM, extracellular matrix   HNF, hepatocyte nuclear factor   GFP, green fluorescent protein   CK-19, cytokeratin-19   GAPDH, glyceraldehyde 3-phosphate dehydrogenase   C/EBP, CCAAT/enhancer binding protein
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号