eIF5A has a function in the elongation step of translation in yeast |
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Authors: | Ana P.B. Gregio |
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Affiliation: | Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University - UNESP, Faculdade de Ciências Farmacêuticas - UNESP, Rodovia Araraquara-Jaú, km 01, Araraquara, SP 14801-902, Brazil |
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Abstract: | The putative translation factor eIF5A is essential for cell viability and is highly conserved throughout evolution. Here, we describe genetic interactions between an eIF5A mutant and a translation initiation mutant (eIF4E) or a translation elongation mutant (eEF2). Polysome profile analysis of single and double mutants revealed that mutation in eIF5A reduces polysome run-off, contrarily to translation initiation mutants. Moreover, the polysome profile of an eIF5A mutant alone is very similar to that of a translation elongation mutant. Furthermore, depletion of eIF5A causes a significant decrease in total protein synthesis and an increase of the average ribosome transit time. Finally, we demonstrate that the formation of P bodies is inhibited in an eIF5A mutant, similarly to the effect of the translation elongation inhibitor cycloheximide. Taken together, these results not only reinforce a role for eIF5A in translation but also strongly support a function for eIF5A in the elongation step of protein synthesis. |
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Keywords: | eIF5A Translation elongation eEF2 Ribosome transit time EF-P Hypusine |
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