Targeted disruption of the mouse Lipoma Preferred Partner gene |
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Authors: | Hilke BVK Vervenne Koen RMO Crombez Veerle Janssens Marleen MR Petit |
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Institution: | a Laboratory for Molecular Oncology, Department of Human Genetics, Herestraat 49, Box 602, K.U.Leuven, Leuven, Belgium b Protein Phosphorylation and Proteomics Laboratory, Department of Molecular Cell Biology, K.U.Leuven, Leuven, Belgium |
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Abstract: | LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp−/− females. Fertility of Lpp−/− males was proven to be normal, however, females from Lpp−/− × Lpp−/− crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp−/− mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp−/− mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter. |
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Keywords: | Zyxin LPP Lipoma Preferred Partner TRIP6 ZRP-1 Knockout Migration Fertility Female lethality Aberrant pregnancy |
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