Expression and localization of regenerating gene I in a rat liver regeneration model |
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Authors: | Jingshu Wang Xiaoping Zhou Li Ma Yukio Koizumi Toshihiro Sugiyama |
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Affiliation: | a Department of Biochemistry, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan b Department of Advanced Biological Sciences for Regeneration (Kotobiken Medical Laboratories), Tohoku University, Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan |
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Abstract: | Regenerating gene (Reg) I has been identified as a regenerative/proliferative factor for pancreatic islet cells. We examined Reg I expression in the regenerating liver of a rat model that had been administered 2-acetylaminofluorene and treated with 70% partial hepatectomy (2-AAF/PH model), where hepatocyte and cholangiocyte proliferation was suppressed and the hepatic stem cells and/or hepatic progenitor cells were activated. In a detailed time course study of activation of hepatic stem cells in the 2-AAF/PH model, utilizing immunofluorescence staining with antibodies of Reg I and other cell-type-specific markers, we found that Reg I-expressing cells are present in the bile ductules and increased during regeneration. Reg I-expressing cells were colocalized with CK19, OV6, and AFP. These results demonstrate that Reg I is significantly upregulated in the liver of the 2-AAF/PH rat model, accompanied by the formation of bile ductules during liver regeneration. |
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Keywords: | Regenerating gene Liver regeneration Hepatic stem cell Oval cell Hepatic progenitor cell Partial hepatectomy Bile ducts 2-AAF/PH |
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