A comparison of shoot regeneration from protoplasts and leaf discs of different genotypes of the cultivated tomato |
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| Authors: | M. M. C. Tan C. M. Colijn-Hooymans W. H. Lindhout A. J. Kool |
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| Affiliation: | (1) Department of Genetics, Free University, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands;(2) Institute for Horticultural Plant Breeding, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands;(3) Present address: Zaadunie B.V. Plant Biotechnology Division, P.O. Box 26, NL-1600 AA Enkhuizen, The Netherlands |
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| Abstract: | Summary Shoot regeneration from leaf discs and leaf mesophyll protoplasts of 11 genotypes of Lycopersicon esculentum (the cultivated tomato), were compared. In both regeneration procedures genotypic differences were observed between inbred lines, and also between F1 hybrids and their parental lines. In the tested hybrid genotypes no heterosis effect with respect to shoot regeneration capacity was observed. A correlation between shoot regeneration from leaf discs and from leaf mesophyll protoplasts was apparent in the tested genotypes. This suggests that using the described procedure, shoot regeneration from leaf discs can be usef for rapid pre-screening for regeneration capacity from protoplasts of tomato genotypes. |
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| Keywords: | Tomato Protoplasts Leaf disc Shoot regeneration Inheritance |
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