STUDIES ON SOLUBLE PROTEINS RELEASED FROM THE SYNAPTIC VESICLES OF RAT BRAIN CORTEX |
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| Authors: | T Matsuda K Saito S Katsuki F Hata H Yoshida |
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| Institution: | Department of Pharmacology, Faculty of Medicine, Osaka University, Osaka, Japan |
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| Abstract: | Abstract— The soluble proteins released from the synaptic vesicles of rat cerebral cortex were studied. One fraction (D4) of these proteins was released in parallel with release of acetylcholine when synaptic vesicles were incubated at 37°C for 10 min in isotonic medium. Another fraction (Dj) was liberated from synaptic vesicles when their membranes were ruptured by mild treatment under hyposmotic conditions and freeze-thawing after release of D1 fraction. Fractions D1 and D2 contained 12 and 9 per cent, respectively, of the total protein in the synaptic vesicles. Some properties of these fractions were investigated by zone electrophoresis and ultracentrifugation, and by measuring their binding capacities for 14C]acetylcholine and various enzyme activities related to acetylcholine metabolism. |
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