Rapid estimation of small amounts of formaldehyde liberated during periodate oxidation of a sialoglycoprotein |
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Authors: | G Durand J Feger M Coignoux J Agneray M Pays |
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Institution: | 2. Laboratoire de Chimie Analytique, U.E.R. des Sciences Pharmaceutiques, 1, rue Vaubenard, 14000—Caen—France |
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Abstract: | We describe a method in order to estimate the amount of formaldehyde liberated by mild periodate oxidation. This MBTH method is a rapid, reliable, sensitive, and a rather specific procedure. We obtain these results: 1 mole of formaldehyde is produced when 1 mole of metaperiodate is consumed by 1 mole of N-acetyl-neuraminic acid. |
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