Measurement of prostaglandin E3 and other eicosanoids in biologic samples using high pressure liquid chromatography and radioimmunoassay

A method to measure PGE₃ in biologic samples is described. Complete resolution of PGE₃ from PGE₁ and PGE₂ is achieved by reverse- phase high pressure liquid chromatography. Quantification is carried out by radioimmunoassay using an antibody directed against PGE₂ that has high cross-reactivity with PGE₃. Using this method, a marked increase in PGE₃ production by mouse kidney tissue and in rat urine was demonstrated after supplemental feeding of ω-3 fatty acids. This method can also be applied to measurement of 6-keto-PGF_1α and TXB₂ in the same samples.