Simultaneous determination of six penicillins in cows' raw milk by a multiresidue high-performance liquid chromatographic method |
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Authors: | Lambert K Sørensen Birthe M Rasmussen Joe O Boison Lily Keng |
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Institution: | Lambert K. Sørensen, Birthe M. Rasmussen, Joe O. Boison,Lily Keng |
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Abstract: | A high-performance liquid chromatographic method based on C18 solid-phase extraction and ultraviolet detection at 323 nm of analytes derivatized with benzoic anhydride and 1,2,4-triazole mercuric chloride solution was developed for the simultaneous determination of amoxicillin, penicillin G (benzylpenicillin), ampicillin, oxacillin, cloxacillin and dicloxacillin residues in raw milk. The detection limit of the method was 1.3 μg/l for penicillin G; 1.4 μg/l for amoxicillin, oxacillin and cloxacillin, 1.5 μg/l for ampicillin and 2.7 μg/l for dicloxacillin. The mean recovery was 95–102% for amoxicillin, penicillin G and ampicillin, 92–98% for oxacillin and cloxacillin and 87–94% for dicloxacillin, measured by using an internal standard. The relative repeatability standard deviation was 4–9% on level 4–15 μg/l, respectively, 2–7% on level 30–40 μg/l. |
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Keywords: | Benzylpenicillin Amoxicillin Penicillins Ampicillin Oxacillin Cloxacillin Dicloxacillin |
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