| 三种蜘蛛粗毒对NG108—15细胞电压门控钠通道的抑制作用 |
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| 引用本文： | 肖玉成,彭宽,陈湘定,梁宋平. 三种蜘蛛粗毒对NG108—15细胞电压门控钠通道的抑制作用[J]. 生命科学研究, 2001, 5(1): 42-47 |
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| 作者姓名： | 肖玉成 彭宽 陈湘定 梁宋平 |
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| 作者单位： | 湖南师范大学 生命科学学院, |
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| 基金项目： | 国家自然科学基金项目(39771058） |
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| 摘 要： | 利用小鼠神经细胞瘤×大鼠神经胶质细胞的杂交细胞NG108-15,通过全细胞记录(whole-cellrecording)模式的膜片钳技术,检验了虎纹捕鸟蛛(Selenocosmiahuwena)、海南捕鸟蛛(Selenocosmia hainana)和广西大疣蛛(Macrothele guangxiasp)的粗毒对NG108-15细胞膜上电压门控TTX敏感型钠电流和延迟整流钾电流的作用.结果表明,三种蜘蛛粗毒对外向延迟整流钾电流没有明显作用,但对TTX敏感型的快钠电流表现出较强的抑制效应.抑制效应呈量效关系.三种粗毒抑制钠电流的EC
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| 关 键 词： | 蜘蛛 NG108－15 钠电流 全细胞记录 钠通道阻断活性 蛛毒 |
| 文章编号： | 1007-7847（2001）01-0042-06 |
| 修稿时间： | 2000-10-10 |
Inhibition ofVoltage-gated Sodium Channel in NG108-15 by the Venoms from Three Kinds of Spider |
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| XIAO Yu-cheng,PENG Kuan,CHEN Xiang-ding,LIANG Song-ping. Inhibition ofVoltage-gated Sodium Channel in NG108-15 by the Venoms from Three Kinds of Spider[J]. Life Science Research, 2001, 5(1): 42-47 |
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| Authors: | XIAO Yu-cheng PENG Kuan CHEN Xiang-ding LIANG Song-ping |
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| Abstract: | Using whole cell patch clamp technology, the effects of the venoms from three kinds of spider (Selenocosmia huwena, Selenocosmia hainana and Macrothele guangxia sp) on voltage activated TTX sensitive(TTX S) Na current and delay rectified K current of undifferentiated NG108 15 were observed. The results showed that they had no evident effect of outword delay rectified K current,while they could inhibit TTX S fast Na current.The inhibitions were dose dependent.The EC 50 values of them were approximate 3.4 mg/L( Selenocosmia huwena ); 1.8 mg/L( Selenocosmia hainana );11.0 mg/L( Macrothele guangxia sp),respectively. |
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| Keywords: | spider NG108 15 sodium current whole cell recording |
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