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Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure
Authors:F Michel  A Jacquier  B Dujon
Institution:Centre de Génétique moléculaire, Laboratoire Propre du Centre National de la Recherche Scientifique, associé à l''Université Pierre et Marie Curie, 91190 Gif sur Yvette, France
Abstract:The complete sequences of nine Saccharomyces cerevisiae mitochondrial introns, six of which carry long open reading frames, have already been published. We have recently determined the sequence of an intron in the large ribosomal mitochondrial RNA of Kluyveromyces thermotolerans (Jacquier et al., in preparation), which we found to be closely related to its S. cerevisiae counterpart. This latter result prompted us to undertake a systematic search for possible homologous elements in the other, available sequences with the help of an original computer program. A previously unsuspected wealth of evolutionarily conserved sequences and secondary structures was thus uncovered. Seven at least of the available sequences may be folded up into elaborate secondary structure models, the cores of which are nearly identical. These models result in bringing together the exon-intron junctions into relatively close spatial proximity and looping out either all or most of the sequences in open reading frame, when present. These results and their possible implications with respect to the mechanism of splicing are discussed in the light of available genetic and biochemical data.
Keywords:introns mitochondriaux  homologies de séquence  structure secondaire des RNAs  phases de lecture introniques  mitochondrial introns  sequence homologies  RNA secondary structure  intronic reading frames
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