Kinetics of protease hydrolysis of extended peptide substrates: measurement by flow-injection analysis |
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| Authors: | G L Chong L C Davis G R Reeck |
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| Affiliation: | Department of Biochemistry, Kansas State University, Manhattan 66506. |
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| Abstract: | A flow-injection analysis (FIA) system was developed to study the enzyme-catalyzed hydrolysis of synthetic peptides, each of which contained one scissile bond. The concentrations of alpha-amino groups in reactions mixtures were determined by FIA with o-phthalaldehyde as a fluorescence reagent. The method allows a rapid, precise, and sensitive determination of kinetic constants for proteases acting on extended peptide substrates. |
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