Novel Cysteine-Rich Peptides from Digitaria ciliaris and Oryza sativa Enhance Tolerance to Cadmium by Limiting its Cellular Accumulation |
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Authors: | Kuramata Masato; Masuya Shuichi; Takahashi Yoshihiro; Kitagawa Etsuko; Inoue Chihiro; Ishikawa Satoru; Youssefian Shohab; Kusano Tomonobu |
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Institution: | 1Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi, 980-8577 Japan
2Akita Agricultural Experiment Station, Akita Prefectural Agriculture, Forestry and Fisheries Research Center, 34-1 Aikawa, Yuwa, Akita, 010-1231 Japan
3Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba, Sendai, Miyagi, 980-8579 Japan
4Department of Environmental Chemistry, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-0856 Japan
5Faculty of Biological Resources, Akita Prefectural University, 241-7 Kaidobata Nishi, Akita, 010-1095 Japan |
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Abstract: | By means of functional screening using the cadmium (Cd)-sensitiveycf1 yeast mutant, we have isolated a novel cDNA clone, DcCDT1,from Digitaria ciliaris growing in a former mining area in northernJapan, and have shown that it confers Cd tolerance to the yeastcells, which accumulated almost 2-fold lower Cd levels thancontrol cells. The 521 bp DcCDT1 cDNA contains an open readingframe of 168 bp and encodes a deduced peptide, DcCDT1, thatis 55 amino acid residues in length, of which 15 (27.3%) arecysteine residues. Five DcCDT1 homologs (here termed OsCDT1–OsCDT5)have been identified in rice, and all of them were up-regulatedto varying degrees in the above-ground tissues by CdCl2 treatment.Localization of green fluorescent protein fusions suggests thatDcCDT1 and OsCDT1 are targeted to both cytoplasmic membranesand cell walls of plant cells. Transgenic Arabidopsis thalianaplants overexpressing DcCDT1 or OsCDT1 displayed a Cd-tolerantphenotype and, consistent with our yeast data, accumulated loweramounts of Cd when grown on CdCl2. Collectively, our data suggestthat DcCDT1 and OsCDT1 function to prevent entry of Cd intoyeast and plant cells and thereby enhance their Cd tolerance. |
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Keywords: | Cadmium - Cysteine-rich peptide - Digitaria ciliaris - Oryza sativa - Tolerance - Transgenic plant |
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