Biosynthesis of acyclic methyl branched polyunsaturated hydrocarbons inPseudomonas maltophilia |
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Authors: | Yu Suen Gunther U Holzer Jerry S Hubbard Thomas G Tornabene |
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Institution: | (1) School of Applied Biology, Georgia Institute of Technology, 30332 Atlanta, GA, U.S.A.;(2) Research Center for Biotechnology, Georgia Institute of Technology, 30332 Atlanta, GA, U.S.A. |
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Abstract: | Summary The hydrocarbon composition ofPseudomonas maltophilia was determined by gas chromatography-mass spectrometry. Mono-, di- and tri-unsaturated alkenes were identified with a predominance of polyunsaturated components. The carbon chains of the alkenes contained single methyl branches iniso andanteiso position and double methyl branches in theiso-iso andanteiso-anteiso configurations. The composition of the hydrocarbons from cells grown in synthetic media enriched with amino acids or volatile fatty acids demonstrated that the probable precursors incorporated into individual hydrocarbons were branched and normal fatty acid chains in the range from C3 to C16. The probable fatty acid precursors which were connected together to form the major triunsaturated hydrocarbon chains were two monounsaturated chains, whereas the major liunsaturated chains resulted from condensation of saturated and monounsaturated chains. The probable precursors for the major monounsaturated hydrocarbons were C14 (C15) and C16 (C15) fatty acids. The accumulation of hydrocarbons was not detected until the cells were in the late exponential phase of growth; the maximal levels were reached at the mid-stationary phase of growth. |
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Keywords: | Biosynthesis Fatty acid Hydrocarbon Lipid Pseudomonas |
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