Embryo sac isolation inArabidopsis thaliana: A simple and efficient technique for structure analysis and mutant selection |
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Authors: | J Wei M -X Sun |
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Institution: | (1) Key Laboratory of MOE for Plant Development Biology, Wuhan University, 430072 Wuhan, P.R. China |
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Abstract: | Arabidopsis thaliana has been widely used as a model plant in gene function analysis. However, its tiny flower and curved embryo sac make it difficult
to study gene expression during megagametogenesis, fertilization, and early embryogenesis, especially in the screening of
mutants from those developmental processes. The techniques currently available are sectioning and whole-mount clearing of
ovules; however, sectioning is time consuming and laborious for quantitative analysis, and whole-mount clearing, makes clear
cytological observation impossible. Reported here is a simple and efficient method based on enzymatic isolation of embryo
sacs that enables both quantitative analysis and elaborate cytological observation for gene expression investigation and mutant
screening. |
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Keywords: | Arabidopsis thaliana embryo sac isolation embryogenesis mutant selection |
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