Acute stress disrupts temporal patterns of behavioral and biochemical parameters of rats |
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| Authors: | Andressa Souza Bernardo Carraro Detanico Liciane Fernandes Medeiros Carla de Oliveira Vanessa Leal Scarabelot Stefania Giotti Cioato |
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| Affiliation: | 1. Post-Graduate Program in Medicine: Medical Sciences – Medicine School, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;2. Pharmacology of Pain and Neuromodulation Laboratory: Pre-clinical Researchs, Department of Pharmacology, Universidade Federal do Rio Grande do Sul, ICBS, Porto Alegre, Brazil;3. Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil;4. Post-Graduate Program in Health and Human Development, Universidade La Salle, Canoas, Brazil;5. Pharmacology of Pain and Neuromodulation Laboratory: Pre-clinical Researchs, Department of Pharmacology, Universidade Federal do Rio Grande do Sul, ICBS, Porto Alegre, Brazil |
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| Abstract: | This study aimed to answer these questions: (i) Can the temporal patterns of locomotor and exploratory activities and anxiety-like behavior be disrupted by a single session of stress? (ii) Can the temporal patterns of corticosterone, melatonin, and glucose be disrupted by a single session of stress and how long does the effect of stress upon their circadian rhythm? Adult male Wistar rats were exposed to restrain stress at four Zeitgeber Times and subdivided according to evaluation times of behavioral (open field test and elevated plus-maze test) and biochemistry (corticosterone, melatonin, and glucose levels in serum) parameters. There were temporal patterns within a course of 24 h in anxiety-like behaviors and glucose levels. Circadian rhythm to corticosterone and melatonin levels was confirmed and a single session of stress can disrupt these temporal patterns. We showed an immediate disruptive effect of stress in biomarkers levels and a delayed appearance of behavioral and locomotion pattern disruption. |
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| Keywords: | Restraint stress circadian rhythm biomarkers locomotor activity anxiety-like behavior and rat |
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