Dynamic feeding low and high methionine diets affect the diurnal rhythm of amino acid transporters and clock related genes in jejunum of laying hens |
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| Authors: | Liu Yi-lin Zhang Ke Wan Dan Zhou Xi-hong Ruan Zheng |
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| Institution: | 1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China;2. National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China;3. Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China;4. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China;5. State Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang, China;6. State Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang, China |
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| Abstract: | This study was conducted to investigate effects of dynamic feeding low and high methionine (Met) diets on circadian clock gene expression in jejunum of laying hens. A total of 180 laying hens (Brown Hy-line, 41 weeks old) were allocated into 3 groups with 6 replicates each. The CON group received diet containing 0.30% Met, LH group received low Met diet (0.27% Met) at 07:30 h and high Met diet (0.33% Met) at 15:30 h, while HL group received high Met diet at 07:30 h and low Met diet at 15:30 h. After 10 weeks, jejunum samples were collected at 4 h intervals in a daily cycle initial starting at 07:30 h before feeding. Results showed that dynamic feeding of Met significantly affected the wave of mRNA expression of B0AT1, LAT1, SNAT2, CAT-2, and y+LAT2 by feeding regime during a day (P<0.05). Dynamic feeding of Met also influenced the fluctuation of relative mRNA expression of Clock, Bmal1, Cry1, Cry2, Per2, and Per3 significantly in hens during a day (P<0.05). These results demonstrated that dynamic feeding different Met level diets of hens affected the rhythmic fluctuation of amino acid transporters and may affected intestinal function by influencing the clock-related genes further. |
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| Keywords: | Dynamic feeding methionine diurnal rhythm transporter clock |
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