Analysis of unique variable region of a plant root inducing plasmid, pRi1724, by the construction of its physical map and library. |
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Authors: | K Moriguchi Y Maeda M Satou M Kataoka N Tanaka K Yoshida |
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Affiliation: | Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan. |
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Abstract: | Ri plasmids in Agrobacterium rhizogenes specifically induce the hairy root syndrome on various dicotyledonous plants. Its T-DNA transfer system as well as those of Ti plasmids have successfully provided the fundamental technique to introduce exogenous genes into plants. To study the Ri genome structure, we constructed a complete BamHI physical map and a lambda library of pRi1724 of A. rhizogenes strain 1724. By using these, we carried out the complete sequence of the 74-kb region between the right border of T-DNA and tra operon, which is the highly variable region (VAR) among Ri and Ti plasmids. As a result, we found three kinds of putative ABC-type transport operons, histidine utilization operon, glycerol utilization operon and two chemoreceptor genes. In addition, a virulence-related gene, tzs was located independently of the vir region. |
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Keywords: | Ri plasmid Agrobacterium rhizogenes Physical map VAR (variable region) |
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