In vivo chain elongation of hepatic DNA: 1-beta-D-arabinofuranosyl cytosine sensitive steps. |
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Authors: | S Rajalakshmi D S Sarma |
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Affiliation: | Fels Research Institute and Departments of Biochemistry and Pathology, Temple University School of Medicine Philadelphia, Pa. 19140 USA |
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Abstract: | The chain elongation of rat liver DNA following partial hepatectomy was studied using alkaline sucrose gradients. DNA made in 5 min was less than 4 × 107 daltons and that made in 30 min was heterodisperse and by 4 hr 75% of the DNA became larger than 1 × 109 daltons. Administration of 1-β-D-arabinofuranosyl cytosine (ara-C) 5 min after thymidine-3H injection inhibited the chain elongation, whereas if given 30 minutes after thymidine-3H pulse did not inhibit the chain elongation. Thus the chain elongation of rat liver DNA consists of at least two steps 1) a step sensitive to ara-C involving nucleotides addition and 2) the other insensitive to ara-C and probably involving ligation of polynucleotide chains. |
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