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A Fine Physical Map of Arabidopsis thaliana Chromosome 5: Construction of a Sequence-ready Contig Map |
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| Authors: | Kotani, Hirokazu Sato, Shusei Fukami, Masanobu Hosouchi, Tsutomu Nakazaki, Naomi Okumura, Satomi Wada, Tsuyuko Liu, You-Guang Shibata, Daisuke Tabata, Satoshi |
| Affiliation: | 1Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292, Japan 2Chiba Prefectural Agricultural Experimental Station Midori-ku, Chiba 266, Japan 3Mitsui Plant Biotechnology Research Institute TCI D-21, Sengen 2-1-6, Tsukuba, Ibaraki 305, Japan |
| Abstract: | A fine physical map of Arabidopsis thaliana chromosome 5 wasconstructed by ordering the clones from YAC, P1, TAC and BAClibraries of the genome using the sequences of a variety ofgenetic and EST markers and terminal sequences of clones. Themarkers used were 88 genetic markers, 13 EST markers, 87 YACend probes, 100 YAC subclone end probes, and 390 end probesof P1, TAC and BAC clones. The entire genome of chromosome 5,except for the centromeric and telomeric regions, was coveredby two large contigs 11.6 Mb and 14.2 Mb long separated by thecentromeric region. The minimum tiling path of the chromosomewas constituted by a total of 430 P1, TAC and BAC clones. Themap information is available at the Web site http://www.kazusa.or.jp/arabi/. |
| Keywords: | Arabidopsis thaliana chromosome 5 physical map contig map |
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