Analysis of theD-region products ofH-2444using monoclonal antibodies reveals the expression of a new class I-like molecule |
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Authors: | Saswati Chatterjee-Das Erik P Lillehoj Diana M Hernandez John E Coligan David H Sachs |
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Institution: | (1) Transplantation Biology Section, Immunology Branch, National Cancer Institute, National Institutes of Health, 20892 Bethesda, MD, USA;(2) Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892 Bethesda, MD, USA;(3) Transplantation Biology Section, Immunology Branch, National Cancer Institute, NIH, Building 10, Room 41317, 20892 Bethesda, MD, USA |
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Abstract: | To analyze how many D-region-encoded molecules could be detected inH-2
q
, we produced a panel of nine monoclonal antibodies from AKR (KkDk) anti-AKR.M (KkDq) immunizations. All of the Dq region antibodies cross-reacted on Dd and/or Ld, and all except one cross-reacted on Db, confirming the previously observed serologic and amino acid sequence homology between theD-region products ofH-2
d
,H-2
b
, andH-2
q
. All of these monoclonal antibodies precipitated 46 000 dalton molecules from both cell-surface-labeled and biosynthetically labeled BIO.AKM spleen cells, indicating that all were reactive with class I-like molecules. Sequential immunoprecipitation analysis with one of these antibodies, 66-3-5, reveals the presence of a previously unidentified class I-like molecule. Tryptic peptide map analysis reveals that this molecule may be the product of a newly describedH-2D
q
-region gene. |
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Keywords: | |
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