| Affiliation: | (1) Department of Surgery, University of Sydney, 2006 Sydney, NSW, Australia;(2) Department of Pharmacology, University of Sydney, NSW, 2006 Sydney, Australia;(3) Department of Medicine, University of Sydney, 2006, NSW, Sydney, Australia;(4) Sydney Cancer Centre, Royal Price Alfred Hospital, 2050, NSW, Sydney, Australia |
| Abstract: | The 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) calorimetric assay is replacing the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as a fast, one-step assay of cell viability. We have observed that evaporation of the outer wells of a 96 well plate increases the absorbancy by 52% compared to the inner wells. Filling the outer 2 rows of wells with media and replacement of the media prior to addition of the MTS reagent will, however, correct this inaccuracy. |
