Application of response surface methodology to optimization of the fixation of ribonuclease a on glutaraldehyde activated amine silica |
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| Authors: | Jean-Louis Marty Camille-Michel Coste |
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| Institution: | (1) Groupe d'Etudes & de Recherches Appliquées Pluridisciplinaires I.U.T.-G.E.R.A.P., Université de PERPIGNAN, Chemin de la Passio Vella, 66025 PERPIGNAN CEDEX |
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| Abstract: | Summary We have used response surface methodology to study the interactions between various parameters (pH, temperature, enzyme concentration) controlling the immobilization of ribonuclease A on to glutaraldehyde activated-amine Spherosil beads. The optimum activity of immobilized ribonuclease A was observed at pH=9.1. The specific activity of the immobilized enzyme was affected by the pH and by the enzyme concentration. |
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