Fine structure physical mapping of 4S RNA genes on mitochondrial DNA of Saccharomyces cerevisiae. |
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| Authors: | Gert-Jan B. Van Ommen Gert S. P. Groot Piet Borst |
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| Affiliation: | (1) Section for Medical Enzymology and Molecular Biology, Laboratory of Biochemsitry, University of Amsterdam, Kruislaan 320, Amsterdam, The Netherlands;(2) Section for Medical Enzymology and Molecular Biology, Jan Swammerdam Institute, Eerste Constantijn Huygensstraat 20, Amsterdam, The Netherlands |
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| Abstract: | Summary We have localized the genes for mitochondrial 4S RNA on the physical map of themtDNA of severalSaccharomyces cerevisiae strains by hybridization of iodinated 4S RNA to the restriction fragments obtained with endonucleasesHindII+III,EcoRI andHapII. The data indicate that 5–8 of the 4S RNA genes are dispersed over a large area of the genome whereas the rest (about 18 genes) is located within an area of about 9000 bp in length (about 12% of the genome) between the markers for chloramphenicol and paromomycin resistance (RIB 1 and PAR 1 loci). Within this region a cluster is present of 5 genes on a DNA fragment of 460 bp.Abbreviations Used mtDNA mitochondrial DNA - mtRNA mitochondrial RNA - rRNA ribosomal RNA - tRNA transfer RNA - C, E, P and O cytoplasmically-inherited resistance markers for chloramphenicol, erythromycin, paromomycin and oligomycin, respectively - SSC 150 mM sodium chloride, 15 mM sodium citrate (pH 7.0) - SDS sodium dodecylsulphate - EDTA (sodium)ethylenediaminetetraacetate; TEMED - N,N,N N -tetramethylethylenediamine; (k)bp, (kilo)base pairs - EthBr ethidium bromide |
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